graphene oxide cryo em

General and robust covalently linked graphene oxide

Here we chemically functionalize cryo-EM grids coated with mostly one or two layers of graphene oxide to facilitate affinity capture. The protein of interest is tagged using a system that rapidly forms a highly specific covalent bond to its cognate catcher linked to the grid via a polyethylene glycol (PEG) spacer.

Making Graphene Oxide Grid — Umass Cryo-EM Docs 1.2

 · Making Graphene Oxide Grid¶ Author. Kangkang Song. Contact Date-created. . Last-updated. . Abstract. This protocol is based on MRC LMB one. We modify the protocol based on Xudong Wu s method at Harvard Medical School.

Single particle cryo-EM reconstruction of 52 kDa

 · Immediately before making the cryo-EM specimen the graphene-coated grid was glow-discharged for 10 s at a low level in a Harrick Plasma instrument after its

Graphene Oxide Based Affinity Grids As Sample Supports For

 · Reproducibility Cryo-EM sample grids can be coated with high-quality graphene oxide (GO) films of consistent thickness and with a high degree of coverage. Low-Cost The method relies on low-cost materials solvents and technology.

Nogales LabCryoEMPublications

Avinash Bharat Patel Daniel Toso Audrey Litvak Eva Nogales Efficient graphene oxide coating improves cryo-EM sample preparation and data collection from tilted grids bioRxiv DOI 10.1101/2021.03.08.434344

Nogales LabCryoEMPublications

Avinash Bharat Patel Daniel Toso Audrey Litvak Eva Nogales Efficient graphene oxide coating improves cryo-EM sample preparation and data collection from tilted grids bioRxiv DOI 10.1101/2021.03.08.434344

A simple and robust procedure for preparing graphene-oxide

 · Graphene oxide (GO) sheets have been used successfully as a supporting substrate film in several recent cryogenic electron-microscopy (cryo-EM) studies of challenging biological macromolecules. However difficulties in preparing GO-covered holey carbon EM

Functionalized graphene as cryo-EM supporting film

The oxidized or hydrogenated graphene film improve both the protein adsorption by 2 3 times and orientation distribution as shown in Figure 2(a). In summary we functionalized the graphene with UV/Ozone or the hydrogen plasma treatment and applied this functionalized graphene to cryo-EM.

Graphene Oxide Based Affinity Grids As Sample Supports For

 · Reproducibility Cryo-EM sample grids can be coated with high-quality graphene oxide (GO) films of consistent thickness and with a high degree of coverage. Low-Cost The method relies on low-cost materials solvents and technology.

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General and robust covalently linked graphene oxide

Here we chemically functionalize cryo-EM grids coated with mostly one or two layers of graphene oxide to facilitate affinity capture. The protein of interest is tagged using a system that rapidly forms a highly specific covalent bond to its cognate catcher linked to the grid via a polyethylene glycol (PEG) spacer.

Functionalized graphene as cryo-EM supporting film

the holey carbon films during cryo-EM specimen preparation 2-4 . Recently graphene oxide film is commonly used as supporting film but graphene oxide is electrical insulator too thick when multiple layers are formed and deposition on the grid is usually inconsistent. So in terms of supporting film the

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Graphene Oxide Based Affinity Grids As Sample Supports For

 · Reproducibility Cryo-EM sample grids can be coated with high-quality graphene oxide (GO) films of consistent thickness and with a high degree of coverage. Low-Cost The method relies on low-cost materials solvents and technology.

Nogales LabCryoEMPublications

Avinash Bharat Patel Daniel Toso Audrey Litvak Eva Nogales Efficient graphene oxide coating improves cryo-EM sample preparation and data collection from tilted grids bioRxiv DOI 10.1101/2021.03.08.434344

Journal of Structural BiologyUni Ulm

 · Graphene Graphene oxide Cryo-EM Electron microscopy abstract Graphene oxide is a hydrophilic derivative of graphene to which biological macromolecules readily attach with properties superior to those of amorphous carbon films commonly used in electron micros-copy. The single-layered crystalline lattice of carbon is highly electron transparent

Amino and PEG-amino graphene oxide grids enrich and

 · Cryo-EM samples prepared using traditional methods often suffer from too few particles poor particle distribution strongly biased orientation or damage from the air-water interface. Here we report that functionalization of graphene oxide (GO) coated grids with amino groups concentrates samples on the grid with improved distribution and

Understanding the invisible hands of sample preparation

 · 1 Understanding the invisible hands of sample preparation for cryo-EM Giulia Weissenberger 1 2 Rene J.M. Henderikx 1 2 Peter J. Peters 2 1CryoSol-World Maastricht The Netherlands 2Maastricht Multimodal Molecular Imaging Institute (M4i) Division of Nanoscopy Maastricht University Maastricht The Netherlands These authors contributed equally.

Making Graphene Oxide Grid — Umass Cryo-EM Docs 1.2

 · Making Graphene Oxide Grid¶ Author. Kangkang Song. Contact Date-created. . Last-updated. . Abstract. This protocol is based on MRC LMB one. We modify the protocol based on Xudong Wu s method at Harvard Medical School.

Making Graphene Oxide Grid — Umass Cryo-EM Docs 1.2

 · Making Graphene Oxide Grid¶ Author. Kangkang Song. Contact Date-created. . Last-updated. . Abstract. This protocol is based on MRC LMB one. We modify the protocol based on Xudong Wu s method at Harvard Medical School.

Structural engineering of graphene for high‐resolution

 · Despite the superior properties of graphene the adoption of graphene as a cryo-EM specimen support still faces some challenges in practical applications. First it is difficult to avoid contaminants during graphene synthesis and transfer generating extra background noise. Second it is difficult to guarantee a high coverage of monolayer graphene.

Structural engineering of graphene for high‐resolution

 · Despite the superior properties of graphene the adoption of graphene as a cryo-EM specimen support still faces some challenges in practical applications. First it is difficult to avoid contaminants during graphene synthesis and transfer generating extra background noise. Second it is difficult to guarantee a high coverage of monolayer graphene.

Efficient graphene oxide coating improves cryo-EM

 · Graphene oxide is a near-ideal support layer for cryo-EM. It produces minimal background in cryo-EM images is sufficiently hydrophilic to bind biological macromolecules can be effectively wetted and blots uniformly during sample preparation 9. So far two main methods have been described to adhere graphene oxide onto the surface of holey carbon

cryo-EM

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Simplified Approach for Preparing Graphene Oxide TEM

In this manuscript we report the application of graphene oxide (GO) in the preparation of cryo-electron microscopy (cryo-EM) and transmission electron microscopy (TEM) grids. We treated GO with water and organic solvents such as methanol ethanol and isopropanol separately to isolate significantly large GO monolayer flake to fabricate the grids for cryo-EM and TEM study.

Protein denaturation at the air-water interface and how to

 · The main problem of graphene for cryo-EM is its extreme hydrophobicity. For an even spread of the protein solution the graphene surface has to be made hydrophilic. Graphene oxide is less hydrophobic than graphene but more difficult to apply to EM grids as a monolayer (Boland et al. 2017).

Approaches to altering particle distributions in cryo

 · Graphene oxide is a naturally hydrophilic derivative of graphene and may offer a more convenient alternative to graphene. It is relatively easy to produce graphene oxide-coated grids using standard EM laboratory equipment and in some cases this can also have a dramatic effect on particle orientation distribution and on-grid concentration

Graphene Substrate preparation and introduction

 · Graphene Graphene oxide Cryo-EM Electron microscopy DNA Transmission electron microscopy abstract This technical note describes the transfer of continuous single-layer pristine graphene to standard Quan-tifoil TEM grids. We compare the transmission properties of pristine graphene substrates to those of graphene oxide and thin amorphous carbon

General and robust covalently linked graphene oxide

 · Here we chemically functionalize cryo-EM grids coated with mostly one or two layers of graphene oxide to facilitate affinity capture. The protein of interest is tagged using a system that rapidly forms a highly specific covalent bond to its cognate catcher linked to the grid via a

Stable Self‐Floating Reduced Graphene Oxide Hydrogel

 · Stable Self-Floating Reduced Graphene Oxide Hydrogel Membrane for High Rate of Solar Vapor Evaporation under 1 sun Pengyu Zhuang National Laboratory of Solid State Microstructures College of Engineering and Applied Sciences School of Physics Key Laboratory of Intelligent Optical Sensing and Integration Ministry of Education Nanjing

Approaches to altering particle distributions in cryo

 · Graphene oxide is a naturally hydrophilic derivative of graphene and may offer a more convenient alternative to graphene. It is relatively easy to produce graphene oxide-coated grids using standard EM laboratory equipment and in some cases this can also have a dramatic effect on particle orientation distribution and on-grid concentration

Graphene Oxide Based Affinity Grids As Sample Supports For

Simplified Approach for Preparing Graphene Oxide TEM

In this manuscript we report the application of graphene oxide (GO) in the preparation of cryo-electron microscopy (cryo-EM) and transmission electron microscopy (TEM) grids. We treated GO with water and organic solvents such as methanol ethanol and isopropanol separately to isolate significantly large GO monolayer flake to fabricate the grids for cryo-EM and TEM study.

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 ·  cryo-EM 8130 19 38 CryoEM amorphous carbon layer Graphene Graphene Oxide